In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics

• Wolfgang G. Kreyling,
• Stefanie Fertsch-Gapp,
• Martin Schäffler,
• Blair D. Johnston,
• Nadine Haberl,
• Christian Pfeiffer,
• Jörg Diendorf,
• Carsten Schleh,
• Stephanie Hirn,
• Manuela Semmler-Behnke,
• Matthias Epple and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180

• dynamical change during AuNP translocation in fluids and across membranes within the organism. Keywords: biokinetics; gold nanoparticles; protein corona; protein–nanoparticle conjugate; serum protein binding; surface modification; Introduction Like any foreign material that enters into the organism

• surface and found that in case of the hydrophobic particles the coverage was rather complete whereas only a very tiny surface percentage of the hydrophilic particles was covered (Table 1). Serum protein binding during in vitro incubation of differently sized AuNP In a second attempt we investigated the

• , surface area and curvature of the AuNP [7]. Serum protein binding of 5 nm sized AuNP with different surface modifications In our next attempt we hypothesized that the configuration of the protein corona (composition, size, geometric orientation) depends on the surface ligands present on the AuNP